SFit is complete and easy to use data analysis software to study complex 3D rapid kinetics data. Typically these data consist of an optical measurement, e.g. absorbance, as a function of both reaction time and wavelength. They may be obtained from transient recordings of this optical signal with fast diode array spectrometers or alternatively are reconstructed from a collection of single wavelength transient kinetic traces.
Raw data form large 3D arrays whose analysis requires sophisticated methods such as included in SFit.
SFit includes the following procedures:
- Data handling and initial treatment
- Singular Value Decomposition (SVD)
- Global multiexponential fitting of the SVD treated data
- Introduction and evaluation of kinetic models